THE STATE OF BIOLOGICAL MARKERS IN WISTAR RATS EXPOSED TO LEAD AND TREATED WITH Allium triquetrum L.
| dc.contributor.author | KAHALERRAS Labiba | |
| dc.date.accessioned | 2022-12-01T14:16:51Z | |
| dc.date.available | 2022-12-01T14:16:51Z | |
| dc.date.issued | 2022-02-24 | |
| dc.description.abstract | Our study aims to evaluate the protective effect of bulbs (B) and leaves (L) of wild garlic aqueous extracts (Allium triquetrum L.) on the reproductive, hepatic, and renal functions induced by lead acetate (Pb) in Wistar rats. Simultaneously, an in vitro study was affected including phytochemical screening, chromatogram (HPLC), and antioxidant activity of A. triquetrum. The in vivo experiment was realized using male rats that divided into 18 groups; the control (C), the Pb (500mg/Kg BW), the positive controls of B and L at different concentrations (2, 3, 4, and 6 g/Kg BW), in addition to the mixtures of each of Pb-B (Pb-B1, Pb-B2, Pb-B3, Pb-B4) and Pb-L (Pb-L1, Pb-L2, Pb-L3, Pb-L4). After 21 days of treatment, Sperm characteristics were evaluated by CASA system, plasma testosterone, and biochemical parameters, as well as testicular, epididymal, hepatic, and renal oxidative stress markers. The phytochemical screening proved that the extracts of B and L were rich in various compounds (polyphenols, flavonoids, and tannins), while the HPLC profile demonstrated that leaves contain more rutin, and, isoquercetin. Moreover, leaves extracts showed better antioxidant activity (DPPH) than bulbs. The in vivo results revealed a significant decrease in the weight of testicles and epididymis, sperm concentration, motility, testosterone, velocity, vitality, round cells, total proteins, albumin, GSH, and GPx level in Pb-treated rats compared to the control, alongside a significant increase in liver and kidney weights, plasma levels of AST, ALT, ALP, urea, creatinine, uric acid, and MDA tissue. However, the co-administration of A. triquetrum extracts (Pb-B and Pb-L) displayed a significant difference in the levels of all previous markers compared to the Pb-group, in a dependant dose manner. In conclusion, aqueous extracts of A. triquetrum bulbs and leaves have the potential to attenuate the repro-toxicity, hepatotoxicity, and nephrotoxicity of Pb through the modulation of the most studied markers in Wistar rats. | |
| dc.format | ||
| dc.identifier.uri | https://dspace.univ-annaba.dz//handle/123456789/731 | |
| dc.language.iso | en | |
| dc.publisher | Université Badji Mokhtar Annaba | |
| dc.subject | allium triquetrum L; Pb; Repro-toxicité; Hépatotoxicité; Néphrotoxicité; Protection; Rat | |
| dc.title | THE STATE OF BIOLOGICAL MARKERS IN WISTAR RATS EXPOSED TO LEAD AND TREATED WITH Allium triquetrum L. | |
| dspace.entity.type |