Browsing by Author "KAOUDOUNE, Chahrazed"

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    Effect of oxidative stress on some target organs and their protection by compound of plant original
    (Université Badji Mokhtar Annaba, 2025) KAOUDOUNE, Chahrazed
    Allium sphaerocephalon L. (AS) is a wild-growing plant frequently utilized as an alternative to onion. The present study focuses on estimation of chemical analysis and antioxidant properties of plant (AS), Furthermore, the study of the preventive action against stomach ulcers induced by 70% ethanol in vivo, as well as the hepatoprotective and nephroprotective from toxicity induced by the administration of doxorubicin (2mg/kg) for 3 times weekly. The antioxidant properties were assessed using five various assays: DPPH radical scavenging assay, reducing power, cupric reducing antioxidant capacity, ferrous ion chelating, and β-carotene bleaching test. These assays demonstrated acceptable antioxidant activities in the hydroethanolic (ASHE) and aqueous (ASAQ) extracts. LC-MS/MS analysis disclosed the existence of 16 phytochemical constituents in the hydroethanolic extract (ASHE), of which 75% are flavonoids, with quinoic acid and acetin (13.808 and 12.616 mg/g of dry extract, respectively) being the major compounds. In other hand, the pretreatment of (ASHE) at dosages of 25, 50, and 100 mg/kg yielded a dose-dependent response enhancement of gastric protection, where the highest dose gave a better protection (99.76%) than ranitidine as a drug reference (90.77%). The ASHE adopted the NO and COX pathways as its protective mechanism in stomach. ASHE (50 and 100 mg/kg) decreased the toxicity of doxorubicin in the liver and kidney by increasing serum levels (AST, ALT, ALP) and (urea, creatinine). Furthermore, ASHE extract improved oxidative stress parameters (GSH, catalase, and MDA) as well as histopathological damage in all organs studied (stomach, liver, and kidney).