IMPACT OF BIOACTIVE MOLECULES ON ADAPTIVE, BEHAVIORAL AND IMMUNE RESPONSES IN WISTAR RAT
No Thumbnail Available
Date
2017
Authors
MATALLAH, Ahlem
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
This study aims at evaluating the behavioral and physiological effects of chronic
administration of β-carboline Harmine and Amitriptyline in rats exposed to CMS procedure.
After 30 days of exposure to CMS procedure, rats were treated with Harmine (15 mg/kg/day,
ip.) or Amitriptyline (20 mg/kg/day, ip.) for 7 days. Afterwards, rats were tested with a
battery of behavioral tests: Annedonia, Open Field (OF), Elevated plus Maze (EPM) and
Light and Dark Box (LDB).
The findings demonstrate that CMS shows behavioral changes during the different tests; a
decrease in the consumption of sweet solutions compared with the control group.
Concerning the data of the effects of CMS in the EPM test, CMS rats demonstrated a decrease
in the number of entries and the time spent in the open arms compared to non-stressed rats.
On the other hand the results show no significant differences on the number of entries and the
time spent in closed arms on CMS rats compared with the control rats.
However, in the OF parameters, we notice a decrease of the time spent in the field center, a
decrease of grooming time, without affecting defecation, crossing and rearing activity in the
stressed group compared with the control group.
The data of the CMS effects on the LDB parameters show a decrease in the number of entries
and the time spent in the light compartment compared with the control group.
Regarding the physiological parameters; the results reveal an increase of ACTH level
compared with the control group. Moreover, CMS alters the immunological parameters by
increasing of IgG, without affecting IgM circulating levels.
The results demonstrate also how that CMS induces a decrease in brain weight and
hypertrophy of adrenal gland.
The chronic treatment with Harmine or Amitriptyline for 1 week reverse anhedonia, OF,
EPM and LDB behaviors, brain and adrenal gland weights, normalized ACTH and
immunoglobulin G (IgG) circulating levels .
In conclusion, treatment with Harmine or Amitriptyline has robust effects in reverting mainly
physiological alterations reliable as indicators of animal depressive disorders.