Bensaci, Narimen2024-05-202024-05-202023https://dspace.univ-annaba.dz//handle/123456789/3462This work gives scientific evidence to the traditional medicinal use of Crataegus azarolus fruits and wild Tuberaria lignosa leaves, highlighting their interest as sources of bioactive compounds that could be used as therapeutic agents. We isolated and characterized polysaccharides from Crataegus azarolus fruits (AZWSP). This latter yielded a 2,19 % fraction, composed of 85.58% total carbohydrates, 4.26% of protein content also contained 5.34% of polyphenols. The preliminary 113 structure features were characterized using FT-IR, Maldi-TOF SM and the H and C NMR analysis. Several mechanisms for antioxidant capacity were examined, including determining -1 relative free radical-scavenging (IC50 = 99 ± 2 µg.ml ), hydroxyl radical scavenging (IC50 = 106 ±-1-13 µg.ml ) and anti-lipid peroxidation activities (IC50 = 127 ± 17 µg.ml), as well as reducing power-1(EC50 =115 µg.ml ). Furthermore, the pulp polysaccharide remained 83% undigested in the artificial digestion process and stimulated the growth o of various isolated lactobacillus species(having probiotic potential), showing a considerable prebiotic ability.enThesis